This book is a completely revised and updated version of the populat Molecular Biology in Histopathology, with added material covering the latest methodologies and now featuring colour plates.
It describes the molecular techniques available and provides an overview of how they can be applied. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology.
Contents:
Chapter 1. Blotting techniques: Methodology and Applications
Chapter 2. In Situ Hybridisation in Histopathology
Chapter 3. DNA Flow Cytometry
Chapter 4. Interphase Cytogenics
Chapter 5. Oncogenes
Chapter 6. Molecular and Immunological Aspects of Cell Proliferation
Chapter 7. Interphase Nucleolar Organiser Regions in Tumour Pathology
Chapter 8. Apoptosis and Cell Senescence
Chapter 9. The Polymerase Chain Reaction
Chapter 10. Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell
Chapter 11. The In Situ Polymerase Chain Reaction
Chapter 12. TaqMan(r) Technology and Real-Time Polymerase Chain Reaction
Chapter 13. Gene Expression Analysis Using Microarrays
Chapter 14. Comparative Genomic Hybridisation in Pathology
Chapter 15. DNA Sequencing and the Human Genome Project
Chapter 16. Monoclonal Antibodies: The Generation and Application of Tools of the Trade Within Biomedical Science
Chapter 17. Proteomics